Research

Research areas at Dept. of Anaesthesia and Intensive Care Medicine, Charles University, 3rd Faculty of Medicine and FNKV University Hospital

We are proud to be a research institution. The department currently has 2 post docs, 5 PhD students, 5 research nurses (3 full time equivalents). Next to theatres and our ICU we have a little cosy lab, equipped with basic stuff, but allowing us to instantly process biological materials such as tissue biopsies, blood sample. We can do cell cultures, high resolution respirometry and acid-base tonometry here, too.

If you are interested in starting a PhD with us, please let us know (email below), we offer a cotutelle program or dual tutorship, too. There are potential opportunities for post-docs, just let us know (frantisek.duska@lf3.cuni.cz, or phone +420608405541).

OXYLAB 

Mitochondrial group

Address: Oxylab, building H, KAR FNKV, 10034, Prague, Czech Republic

Core staff

Dr František Duška, PhD, A/Prof

frantisek.duska@lf3.cuni.cz, PA: Mrs Iva Halešová, karsec@fnkv.cz , tel: +420267162461

Dr Kateřina Jiroutková, PhD

post doc, email: katka.nova@post.cz

Dr Adéla Krajčová, PhD

now Pasteur’s Institute, Paris

Tomáš Urban

PhD student

Dr Petr Waldauf

PhD student

Collaborators

Dr Bob Bakalář

PhD student

Dr Jan Gojda, PhD

Lab of metabolic physiology, Dept. of Medicine II, FNKV University Hospital

Prof. Francis Stephens

University of Exeter United Kingdom

Nils Gunnar Løvsletten, MSci Pharm

Dpt. of Pharmaceutical Biosciences, University of Oslo, Norway

Dr Eva Pokorna, PhD, A/Prof

Institute of Experimental Medicine, Prague

Dr Zudin Puthucheary, PhD

Barts and The London School of Medicine & Dentistry, Queen Mary University of London, United Kingdom

RNDr. Zdeněk Drahota, DrSc

Institute of Physiology of Czechoslovak Academy of Sciences

Research Nurses

studijni.sestry@gmail.cz

Šárka Gregorová

Šárka Marešová

Kateřina Ťopková

Topics & Research Questions

  • Innovative techniques of exercise delivery, such as functional-electrical stimulation-assisted cycle ergometry
  • Bioenergetic failure ant its relation to ICU-acquired weakness and failed ICU outcomes
  • Influence of drugs commonly used in anaesthesia or ICU (such as propofol or antiarrhytmics) onto cellular bioenergetis

Grants

  • Active: Czech Agency For Research in Healthcare AZV 16-28663A (EUR 760 000) Functional Electrical Stimulation‐Assisted Cycle Ergometry in Critically Ill. Principal applicant: F. Duska. This is a randomised controlled trial with muscle physiology studies embedded in it (See protocols in Trials doi: https://ichgcp.net/clinical-trials-registry/NCT02864745)
    • Under evaluation: GACR 20-25779S Effects of antiarrhythmic drugs on bioenergetics in human cardiac cells. Principal applicant: K. Jiroutkova

    Recent publications

    Less Usual Drugs, Less Usual Ways

    Pharmacological Group

    Core staff

    Dr Jiri Malek, PhD, A/Prof

    Dr Milada Halacova MSci Pharm, PhD

    Dr Alice Kurzova

    PhD student

    Dr Veronika Rehorova

    PhD student

    This groups focuses of finding alternative ways of administering old drugs, such as intranasal midazolam or intrathecal morphine, or piloting unconventional new ways of treatments on the border of pharmacology, such as human faecal microbial transplantation for postantibiotic diarhea in the critically ill patients. 

    Ongoing RCTs: Intrathecal Morphine Analgesia vs. Continuous Epidural Analgesia vs. Systemic Analgesia in Colorectal Surgery. (KOLORIT) Principal investigator J. Malek, NCT03007121

    Publications

    • Sliva J, Hess L, Votava M, Malek J. Endogenous ligands of benzodiazepine binding site have inverse agonistic properties. Med Hypotheses. 2013 Dec;81(6):1075-7. doi: 10.1016/j.mehy.2013.10.006. Epub 2013 Oct 16. PubMed PMID: 24183322.
    • Jaček M, Matějčková J, Málek J, Hess L, Samcová E. Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration. J Sep Sci. 2013 Oct;36(20):3366-71. doi: 10.1002/jssc.201300401. Epub 2013 Sep 1. PubMed PMID: 23926127. 
    • Janík V, Skrabalová D, Gürlich R, Málek J. [Irreversible electroporation: local, non-thermal, ablation therapy of malignant tumours]. Cas Lek Cesk. 2013;152(2):67-75. Czech. PubMed PMID: 23641678.

    Carboxylab

    Acid Base research

    Core staff

    Dr František Duška, PhD, A/Prof

    Dr Martin Krbec

    PhD student

    Dr Petr Waldauf

    Collaborators

    Dr Thomas Langer, A/Prof, University of Milan, Italy

    Dr Paul WG Elbers, PhD

    VUMC, The Netherlands

    Dr Morten H. Bestle

    University of Copenhagen, Denmark

    This is a relatively new group that looks into the source of errorrs in acid-base determination, such as irregularities in constants used in acid-base calculation. The projects are performed in very close international collaboration. Don’t get tricked, the Carboxylab has the same address as Oxylab. It is actually the same room. 

    • Ongoing projects: Determination of the Dissociation Constant (Ka) of Plasma and Whole Blood in Septic Patients (ENTERPRISE), see NCT03966664 at clinicaltrials.gov

    Grants

    • Grants 4EU+ Start-up grant Sources of error in acid base determination. Principle investigator F. Duska 
    • Under evaluation: GACR 20-25779S Weak acid dissociation constant in human plasma and its determinants – implications for interpretation of acid-base status assessment Abbreviated name: Ka-Project.  Principal applicant: F. Duska and other project where T. Langer is the PI

    Data science

    Dr Petr Waldauf

    • The data management and analysis using R, Python and Stata
    • The development of electronic Case Report Forms (eCRF) for our clinical trials
    • The customisation of MetaVison clinical information system
    • Publications

    Other collaborative research

    Individuals from our departmens collaborate with other institutions on a range of research projects such as neurobiology of TBI (Dr M Kolar, PhD), non-technical skills and medical simulation (dr Stern, dr. Vesela), investigation of physiology of dying (Dr Schmidt, dr. Waldauf, with a center in Canada) or medical educationalism (Dr A. Le Roy, Dr P. Waldauf – standard setting and analysis of EDIC exam), among others.

    Examples of recent collaborative publications where our employee is the first or senior author:

    • Kolar M, Nohejlova K, Duska F, Mares J, Pachl J. Changes of cortical perfusion in the early phase of subarachnoid bleeding in a rat model and the role of intracranial hypertension. Physiol Res. 2017 Dec 30;66(Supplementum 4):S545-S551. PubMed PMID: 29355383.
    • Kolar M, Nohejlova K, Mares J, Pachl J. Early changes of brain perfusion after subarachnoid hemorrhage – the effect of sodium nitroprusside. Physiol Res. 2016 Dec 22;65(Supplementum 5):S591-S599. PubMed PMID: 28006941.
    • Knor J, Seblova J, Skulec R, Seblova D, Jiri M. The presence of gasping predicts long-term survival in out-of-hospital cardiac arrest patients. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2018 Mar;162(1):32-35. doi: 10.5507/bp.2017.053. Epub 2017 Dec 13. PubMed PMID: 29235579.
    • Waldauf P, Rubulotta F, Sitzwohl C, Elbers P, Girbes A, Saha R, Marsh B, Kumar R, Maggiorini M, Duška F. Factors associated with success in the oral part of the European Diploma in Intensive Care. J Intensive Care Soc. 2017 Nov;18(4):294-299. doi: 10.1177/1751143717712623. Epub 2017 Jul 5. PubMed PMID: 29123559; PubMed Central PMCID: PMC5661790.

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    © Petr Waldauf, KAR, FNKV 2019

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