Address: Oxylab, building H, KAR FNKV, 10034, Prague, Czech Republic

Core staff

Research Nurses

studijni.sestry@gmail.cz

Topics & Research Questions

• Innovative techniques of exercise delivery, such as functional-electrical stimulation-assisted cycle ergometry
• Bioenergetic failure ant its relation to ICU-acquired weakness and failed ICU outcomes
• Influence of drugs commonly used in anaesthesia or ICU (such as propofol or antiarrhytmics) onto cellular bioenergetis

Grants

• Active: Czech Agency For Research in Healthcare AZV 16-28663A (EUR 760 000) Functional Electrical Stimulation‐Assisted Cycle Ergometry in Critically Ill. Principal applicant: F. Duska. This is a randomised controlled trial with muscle physiology studies embedded in it (See protocols in Trials doi: https://ichgcp.net/clinical-trials-registry/NCT02864745)
• Under evaluation: GACR 20-25779S Effects of antiarrhythmic drugs on bioenergetics in human cardiac cells. Principal applicant: K. Jiroutkova

Recent publications

• Petr Waldauf, Jan Gojda, Tomáš Urban, Natália Hrušková, Barbora Blahutová, Marie Hejnová, Kateřina Jiroutková, Michal Fric, Pavel Jánský, Jana Kukulova, Francis Stephens, Kamila Řasová, František Duška. Functional Electrical Stimulation‐Assisted Cycle Ergometry in Critically Ill. Protocol of Randomised Controlled Trial. 
• Urban T, Waldauf P, Krajčová A, Jiroutková K, Halačová M, Džupa V, Janoušek L, Pokorná E, Duška F. Kinetic characteristics of propofol-induced inhibition of electron-transfer chain and fatty acid oxidation in human and rodent skeletal and cardiac muscles. PLoS One. 2019 Oct 4;14(10):e0217254. doi: 10.1371/journal.pone.0217254. eCollection 2019. PMID: 31584947
• Gojda J, Waldauf P, Hrušková N, Blahutová B, Krajčová A, Urban T, Tůma P, Řasová K, Duška F. Lactate production without hypoxia in skeletal muscle during electrical cycling: Crossover study of femoral venous-arterial differences in healthy volunteers. PLoS One. 2019 Mar 1;14(3):e0200228. doi: 10.1371/journal.pone.0200228. eCollection 2019. PubMed PMID: 30822305; PubMed Central PMCID: PMC6396965.
• Krajčová A, Løvsletten NG, Waldauf P, Frič V, Elkalaf M, Urban T, Anděl M, Trnka J, Thoresen GH, Duška F. Effects of Propofol on Cellular Bioenergetics in Human Skeletal Muscle Cells. Crit Care Med. 2018 Mar;46(3):e206-e212. doi: 10.1097/CCM.0000000000002875. PubMed PMID: 29240609.
• Krajčová A, Waldauf P, Anděl M, Duška F. Propofol infusion syndrome: a structured review of experimental studies and 153 published case reports. Crit Care. 2015 Nov 12;19:398. doi: 10.1186/s13054-015-1112-5. Review. PubMed PMID: 26558513; PubMed Central PMCID: PMC4642662.
• Jiroutková K, Krajčová A, Žiak J, Fric M, Gojda J, Džupa V, Kalous M, Tůmová J, Trnka J, Duška F. Mitochondrial Function in an In Vitro Model of Skeletal Muscle of Patients With Protracted Critical Illness and Intensive Care Unit-Acquired Weakness. JPEN J Parenter Enteral Nutr. 2017 Sep;41(7):1213-1221. doi: 10.1177/0148607116657649. Epub 2016 Jun 29. PubMed PMID: 27358332.
• Jiroutková K, Krajčová A, Ziak J, Fric M, Waldauf P, Džupa V, Gojda J, Němcova-Fürstová V, Kovář J, Elkalaf M, Trnka J, Duška F. Mitochondrial function in skeletal muscle of patients with protracted critical illness and ICU-acquired weakness. Crit Care. 2015 Dec 24;19:448. doi: 10.1186/s13054-015-1160-x. PubMed PMID: 26699134; PubMed Central PMCID: PMC4699339.
• Musutova M, Elkalaf M, Klubickova N, Koc M, Povysil S, Rambousek J, Volckaert B, Duska F, Trinh MD, Kalous M, Trnka J, Balusikova K, Kovar J, Polak J. The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes. Front Endocrinol (Lausanne). 2018 Oct 17;9:616. doi: 10.3389/fendo.2018.00616. eCollection 2018. PubMed PMID: 30386299; PubMed Central PMCID: PMC6199370.
• Ziak J, Krajcova A, Jiroutkova K, Nemcova V, Dzupa V, Duska F. Assessing the function of mitochondria in cytosolic context in human skeletal muscle: adopting high-resolution respirometry to homogenate of needle biopsy tissue samples. Mitochondrion. 2015 Mar;21:106-12. doi: 10.1016/j.mito.2015.02.002. Epub 2015 Feb 17. PubMed PMID: 25701243.
• Krajcova A, Ziak J, Jiroutkova K, Patkova J, Elkalaf M, Dzupa V, Trnka J, Duska F. Normalizing glutamine concentration causes mitochondrial uncoupling in an in vitro model of human skeletal muscle. JPEN J Parenter Enteral Nutr. 2015 Feb;39(2):180-9. doi: 10.1177/0148607113513801. Epub 2013 Nov 29. PubMed PMID: 24291738.

Less Usual Drugs, Less Usual Ways

Pharmacological Group

Core staff

This groups focuses of finding alternative ways of administering old drugs, such as intranasal midazolam or intrathecal morphine, or piloting unconventional new ways of treatments on the border of pharmacology, such as human faecal microbial transplantation for postantibiotic diarhea in the critically ill patients. 

Ongoing RCTs: Intrathecal Morphine Analgesia vs. Continuous Epidural Analgesia vs. Systemic Analgesia in Colorectal Surgery. (KOLORIT) Principal investigator J. Malek, NCT03007121

Publications

• Sliva J, Hess L, Votava M, Malek J. Endogenous ligands of benzodiazepine binding site have inverse agonistic properties. Med Hypotheses. 2013 Dec;81(6):1075-7. doi: 10.1016/j.mehy.2013.10.006. Epub 2013 Oct 16. PubMed PMID: 24183322.
• Jaček M, Matějčková J, Málek J, Hess L, Samcová E. Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration. J Sep Sci. 2013 Oct;36(20):3366-71. doi: 10.1002/jssc.201300401. Epub 2013 Sep 1. PubMed PMID: 23926127. 
• Janík V, Skrabalová D, Gürlich R, Málek J. [Irreversible electroporation: local, non-thermal, ablation therapy of malignant tumours]. Cas Lek Cesk. 2013;152(2):67-75. Czech. PubMed PMID: 23641678.

Carboxylab

Acid Base research

Core staff

Collaborators

This is a relatively new group that looks into the source of errorrs in acid-base determination, such as irregularities in constants used in acid-base calculation. The projects are performed in very close international collaboration. Don’t get tricked, the Carboxylab has the same address as Oxylab. It is actually the same room. 

• Ongoing projects: Determination of the Dissociation Constant (Ka) of Plasma and Whole Blood in Septic Patients (ENTERPRISE), see NCT03966664 at clinicaltrials.gov

Grants

• Grants 4EU+ Start-up grant Sources of error in acid base determination. Principle investigator F. Duska 
• Under evaluation: GACR 20-25779S Weak acid dissociation constant in human plasma and its determinants – implications for interpretation of acid-base status assessment Abbreviated name: Ka-Project.  Principal applicant: F. Duska and other project where T. Langer is the PI

Data science

• The data management and analysis using R, Python and Stata
• The development of electronic Case Report Forms (eCRF) for our clinical trials
• The customisation of MetaVison clinical information system
• Publications